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کروماتوگرافی مرا جع: 1-Principles of Instrumental Analysis D.A.Skoog2- Instrumental Analysis C.D.Chiristian and J.G.Oeilly 3- Physical and chemical methods of separation Berg  2 واحد درسی( کارشناسی ارشد) مدرس : دکتر نژاد علی 
Introduction
Plant Pigments
Chlorophylls
Xanthophylls
Martin & synge(1952 Nobel Prize)
A general description chromatography
Stationary phase
Mobile phase

Classification of chromatography
Column chromatography
Planer chromatography
Linear chromatography
Partition coefficient
K = Cs/Cm
Cs , is molar analytical
Cm , solute concentration in the mobile phase
Elution chromatography on columns
Elution is defined as a process whereby a solute is washed through a column by additions of fresh solvent.
Retention time (tR)
Qualitative & Quantitative Chromatography
Chromatograms
If a detector that responds to solute concentration is placed at the end of the column & its signal is plotted as function of time (or of volume of the added mobile phase), a series of peaks is obtained, such a plot , called a chromatogram.
Theories of elution chromatography
The effects of migration rates & zone broadening on resolution
Plate theory
Kinetic theory
L=NH
N=L/H

Retention time
V=L/tR
....